En 60 puntos de la ciudad, se cuantificó el material particulado sobre las hojas ( PMAL) y la respuesta fisiológica a las emisiones vehiculares en cinco especies 

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In the pMAL™ Protein Fusion and Purification System, the cloned gene is inserted into a pMAL vector down-stream from the malE gene, which encodes maltose-binding protein (MBP). This results in the expression of an MBP-fusion protein (1,2,3). The technique uses the strong P tac promoter and the translation initiation signals of MBP to express

The system uses the pMAL vectors which are designed so that insertion of a target gene results in an MBP fusion protein. pMAL-c5X has an exact deletion of the malE signal sequence, resulting in cytoplasmic expression of the fusion protein. pMAL-p5X contains the normal malE signal sequence, which directs the fusion protein through the cytoplasmic membrane. pMAL-p5 fusion proteins capable … pMAL-pIII contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322; in ad-dition, it contains an M13 origin of replication (2). The multiple cloning site (MCS) from pMAL-p2X is present in pMAL-pIII but is not used. Enzymes with unique restriction sites are shown in bold type.

Pmal al

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for the LBD from aa377 to aa579 was cloned in the pMAL-c5x vector (Biolabs,  På samma sätt beskriver Brissac et al . visade att anti-EYYLN-antikroppar kan och infördes i pMAL-p2x-plasmiden (NEB) vid EcoRl- och Pstl-kloningsställena. ede75LD1)!&H%5m6skW^aL;;EPze=CjmHU>K%6uySE9rd`-D3smL3  alabama.

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In the pMAL™ Protein Fusion and Purification System, the cloned gene is inserted into a pMAL vector down-stream from the malE gene, which encodes maltose-binding protein (MBP). This results in the expression of an MBP-fusion protein (1,2,3). The technique uses the strong P tac promoter and the translation initiation signals of MBP to express

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The pMAL-c6T Vector provides a method for producing a protein expressed from a cloned gene or open reading frame. The cloned gene is inserted downstream from the malE gene of E. coli, which encodes maltose-binding protein (MBP), resulting in the expression of an MBP fusion protein in the cytoplasm (1,2). Check out the product page for the new Biotinylated PMAL-C8 by clicking here! References: Tribet, C., et al. (1996) Proc Natl Acad Sci U S A 93(26), 15047-15050.
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PMAL >K7GTE0 MSLEKLNEEWKLVQNDFQSLEKIHDEYIQKSRQVSKFQDTAGKAMKHHNYLLKNLRETFR AL >K7GTE7 

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